A local anesthetic, tetracaine, similarly inhibits Ag+ and K+ contractures in frog skeletal muscle.

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Biphasic Potassium Contractures in Frog Muscle Fibers

Potassium-induced contractures were studied in single fibers from the semitendinosus muscle of Rana pipiens. Contractures elicited by solutions containing 60-117 mM potassium and 120 mM chloride were biphasic, consisting of a rapid initial contraction with a duration at 23 degrees C of less than 1 sec followed by a slow response with a duration of many seconds. At 13 degrees C, the initial resp...

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Differential effects of tetracaine on charge movements and Ca2+ signals in frog skeletal muscle

The effects of tetracaine on charge movements and on antipyrylazo III signals monitoring intracellular delta [Ca2+] were compared in cut frog semitendinosus muscle fibers in a single vaseline gap-voltage clamp. Low tetracaine concentrations (25-40 microM) markedly reduced delta [Ca2+] signals and shifted the rheobase. However, they neither influenced charge movement nor that peak delta [Ca2+] v...

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Effects of Tetracaine on Voltage-activated Calcium Sparks in Frog Intact Skeletal Muscle Fibers

The properties of Ca(2+) sparks in frog intact skeletal muscle fibers depolarized with 13 mM [K(+)] Ringer's are well described by a computational model with a Ca(2+) source flux of amplitude 2.5 pA (units of current) and duration 4.6 ms (18 degrees C; Model 2 of Baylor et al., 2002). This result, in combination with the values of single-channel Ca(2+) current reported for ryanodine receptors (...

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Frog Skeletal Muscle

Autoradiographs consisting of a 1000 A thick tissue section and a 1400 A thick emulsion film have been prepared from frog toe muscles labeled with Ca 45. The muscles had been fixed with an oxalate-containing osmium solution at rest at room temperature, at rest at 40°C, during relaxation following K+ depolarization or after prolonged depolarization. From 6 to 39 per cent of K+ contracture tensio...

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Sparks in Frog Skeletal Muscle

Calcium sparks are brief, highly localized elevations of [Ca 2 ], which arise spontaneously or can be evoked by depolarization in many types of muscle cells. The Ca 2 is released into the cytoplasm from intracellular stores (the SR) through the SR Ca 2 release channels (the RYR). Since their discovery (in cardiac muscle; Cheng et al., 1993), Ca 2 sparks have attracted substantial attention. The...

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ژورنال

عنوان ژورنال: The Japanese Journal of Physiology

سال: 1987

ISSN: 1881-1396,0021-521X

DOI: 10.2170/jjphysiol.37.995